ANALYTICAL
CHEMISTRY DIVISION
COMMISSION ON SEPARATION METHODS IN ANALYTICAL CHEMISTRY*
Retention Parameters in Chromatography (IUPAC Recommendations 2001)
Part A. Hold-up Volume Concept in Column Chromatography
José Antonio García Domínguez1 and José
Carlos Díez-Masa2
1Institute of Physical Chemistry "Rocasolano",
CSIC, Serrano, 119. 28006 Madrid, Spain; 2Institute of Organic Chemistry,
CSIC, Juan de la Cierva, 3. 28006 Madrid, Spain
Part B. Retention parameters in gas chromatograpy
Vadim A. Davankov
Nesmeyanov-Institute of Organo-Element Compounds, Russian
Academy of Sciences, Moscow, 119991, Vavilov Str. 28, Russia
Abstract: The paper presents a revision of terms in the IUPAC
"Nomenclature for Chromatography", Pure and Applied Chemistry,
65, 819-872, 1993. The terms revised pertain to hold-up volumes in gas,
liquid, and supercritical-fluid chromatography, as well as to basic
retention parameters, especially in gas chromatography. A number of
related and derived definitions are described, including definitions
of the terms "chromatographic process" and "chromatographic
phase system". A number of the original terms were found to be
misleading or superfluous, including such terms as corrected retention
time, net retention time, total retention volume (time), and specific
retention volume at 0 °C, and their use is strongly discouraged
In Part A, the concept of the hold-up volume in chromatography is discussed.
The paper also compares methods described in the literature to determine
the hold-up volume.
In Part B, retention parameters in gas chromatography are discussed
with the aim of (i) emphasizing the physical meaning of the terms and
(ii) specifying the temperatures and pressures for the terms for gas
volumes and flow rates.
The appendix presents revised recommendations for the terminology of
some items, as well as those that are not recommended.
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