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Pure Appl. Chem.
75(10), 1371-1381, 2003
Pure and Applied Chemistry
Vol. 75, Issue 10
Molecular structure and dynamics of proteins in solution: Insights derived
from high-resolution NMR approaches*
D.A.Torchia and R. Ishima
Molecular Structural Biology Unit, National Institute
of Dental and Craniofacial Research, National Institutes of Health,
Bethesda, MD 20892, USA
Abstract: This article reviews heteronuclear NMR techniques
that are used to characterize the 3D structures and internal molecular
dynamics of proteins in solution. Applications to several proteins that
have been studied in our laboratory are presented. Topics discussed
include sequential assignments, the use of nuclear Overhauser enhancement
spectroscopy (NOESY) and residual dipolar coupling restraints to determine
protein structure, and the use of relaxation measurements to obtain
information about protein internal motions. Also discussed are recent
computational approaches directed at increasing the efficiency of structure
determination, as well as new methods for studying large proteins.
*Lecture presented at the IUPAC Polymer Conference
on the Mission and Challenges of Polymer Science and Technology (IUPAC
PC2002), Kyoto, Japan, 2-5 December 2002. Other presentations are published
in this issue, pp. 1359-1402.
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